In MCF7 cells, treatment with either single agent reduced c Myc and Bcl 2 and increased p21 expression [Only registered and activated users can see links. Click Here To Register...] The cells were then washed twice with 2 FBS 1 PBS followed by incubation for 60 minutes with 100 ОјL of secondary anti mouse antibody AlexaFluor 488 at a final concentration of 10 Ојg mL
